Histomorphological Observations on the Parathyroid Glands in White Leghorn Birds

Parathyroid glands from laying hens were also  xed in percent glutaraldehyde in phosphate buffer (0.1 M, 7.3 pH) for araldite embedding and used for electron microscopy. For routine histological observations, sections of 5 to 6 um thickness were cut and stained by, Mayer’s haemalum and eosin-phloxine, Masson’s trichrome, Crossman’s modi cation of Mallory’s triple stain, van Gieson’s method for collagen  bre, Silver, Orcein and Aniline blue method for selective demonstration of elastin, reticulum and collagen, Toluidine blue method for semithin sections, Periodic acid Schiff (PAS) reaction for carbohydrates, PAS with alcian blue for the differentiation of acid mucopolysaccharides. The frozen sections were used for staining in Oil-red ‘0’ in propylene glycol for localizing the lipids and Gomori’s method for localizing acid phosphatase activity (Bancroft and Gamble, 2003).